Tag: NGS data formats
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The Complete Guide to NGS Data Types and Formats: From Raw Reads to Analysis-Ready Files
Master the essential file formats in next-generation sequencing analysis Introduction: Understanding the NGS Data Ecosystem Next-generation sequencing (NGS) has revolutionized biological research by enabling us to read DNA, RNA, and epigenetic modifications at an unprecedented scale. However, with this power comes complexity – NGS workflows generate dozens of different file formats, each serving specific purposes
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Alternative Splicing Analysis ATAC-seq BAM ChIP-seq chromatin accessibility CNV DESeq2 Differential Expression edgeR FASTQ GATK Mutect2 gene expression heatmap HOMER HPC Isoform limma MACS2 MAF miRNA miRNA-seq MSigDB Normalization peak calling RNA-seq SLURM somatic mutations Transcript VCF whole genome sequencing