Tag: samtools fastq
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How to Convert BAM Files Back to FASTQ Files: A Practical Guide for NGS Analysis
Introduction: When and Why You Need BAM-to-FASTQ Conversion The NGS Data Conversion Challenge In next-generation sequencing (NGS) analysis, you’ll encounter data in different formats depending on where you are in your workflow. Sometimes you need to convert between these formats, particularly from BAM (aligned reads) back to FASTQ (raw sequencing reads). Why Do Data Repositories…
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Recent Posts
- How to Analyze Single-Cell ATAC-seq Data — A Complete Beginner’s Guide Part 2: Thorough Quality Control with Signac
- How to Analyze Single-Cell ATAC-seq Data — A Complete Beginner’s Guide Part 1: From FASTQ to Peaks
- How to Choose the Best Genome Aligner for a Specific NGS Dataset — A Beginner’s Guide to Read Mapping Tools
- How to Set Up a Bulk RNA-seq Pipeline on an HPC Cluster — A Complete Beginner’s Guide to Nextflow and nf-core/rnaseq
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Alternative Splicing Analysis ATAC-seq BAM ChIP-seq chromatin accessibility CNV DESeq2 Differential Expression edgeR FASTQ GATK Mutect2 gene expression heatmap HOMER HPC Isoform limma MACS2 MAF miRNA miRNA-seq MSigDB Normalization peak calling RNA-seq SLURM somatic mutations Transcript VCF whole genome sequencing