Tag: FASTQ
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How to Analyze RNAseq Data for Absolute Beginners Part 2: From Fastq to Counts – Best Practices
Introduction The most straightforward way to obtain a count table is to request it directly from your sequencing company or your institution’s sequencing core. This option may involve an additional fee. However, for those eager to learn or save money, let’s walk through the process together. Before we dive in, a quick reminder: If you…
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Adapter Trimming Alternative Splicing Analysis BAM bar plot breast cancer classification cancer subtypes cell-type composition CIBERSORTx Conda Environment Setting Count Differential Expression dotplot Enrichment FASTQ gene expression Gene Expression Quantification ggplot2 GO GSEA immune cell profiling KEGG molecular subtypes MSigDB PAM50 Pathway R Reads Mapping RNA-seq analysis RNAseq analysis for beginners violin plot