Tag: ChIP-seq
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How To Analyze ChIP-seq Data For Absolute Beginners Part 5: Mastering Reproducibility With IDR Analysis
Introduction: Why Reproducibility Is Crucial In ChIP-seq Analysis Chromatin Immunoprecipitation followed by high-throughput sequencing (ChIP-seq) has revolutionized our understanding of protein-DNA interactions across the genome. However, like all experimental techniques, ChIP-seq is subject to both technical and biological variability. This variability creates a fundamental challenge: how do we distinguish genuine protein binding events from experimental…
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How To Analyze ChIP-seq Data For Absolute Beginners Part 4: From FASTQ To Peaks With MACS2
Introduction: Expanding Your ChIP-seq Analysis Toolkit Chromatin immunoprecipitation followed by sequencing (ChIP-seq) has revolutionized our understanding of protein-DNA interactions across the genome. In this fourth installment of our ChIP-seq tutorial series, we explore an alternative approach to peak calling using MACS2 (Model-based Analysis of ChIP-Seq), a widely used and statistically robust tool that complements the…
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How To Analyze ChIP-seq Data For Absolute Beginners Part 3: Differential Binding Analysis and Motif Discovery
Introduction: Moving Beyond Basic Peak Calling In our previous tutorials, we walked through the fundamental process of ChIP-seq analysis, from raw sequencing reads to identifying where a protein of interest binds to DNA. While finding these binding sites (peaks) is a crucial first step, it’s often just the beginning of extracting meaningful biological insights from…
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How To Analyze ChIP-seq Data For Absolute Beginners Part 1: From FASTQ To Peaks With HOMER
Video Tutorial Introduction: Understanding ChIP-seq At the heart of molecular biology lies a fundamental question: how do cells regulate which genes are expressed and when? One of the most powerful techniques to explore this question is Chromatin Immunoprecipitation followed by sequencing, commonly known as ChIP-seq. This tutorial introduces beginners to the fascinating world of ChIP-seq…
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Recent Posts
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Tags
Adapter Trimming Alternative Splicing Analysis ATAC-seq BAM ChIP-seq chromatin accessibility Conda Environment Setting Count DESeq2 Differential Expression edgeR FASTQ gene expression Gene Expression Quantification HOMER HPC Isoform limma MACS2 miRNA miRNA-seq Normalization PCA peak calling R Reads Mapping RNAseq analysis RNAseq analysis for beginners RStudio Transcript