Author: Lei
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How to Analyze RNAseq Data for Absolute Beginners Part 3: From Count Table to DEGs – Best Practices
As we move forward in our RNAseq analysis journey, we’ll be transitioning from the Linux environment to R, a powerful and versatile statistical analysis tool. R is not only a programming language but also a platform widely used in data science, statistical computing, and predictive modeling. Tech giants like Microsoft, Meta, Google, Amazon, and Netflix…
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How to Analyze RNAseq Data for Absolute Beginners Part 2: From Fastq to Counts – Best Practices
Introduction The most straightforward way to obtain a count table is to request it directly from your sequencing company or your institution’s sequencing core. This option may involve an additional fee. However, for those eager to learn or save money, let’s walk through the process together. Before we dive in, a quick reminder: If you…
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How to analyze RNAseq Data for Absolute Beginners Part 1: Environment setup
Introduction RNA sequencing (RNAseq) has revolutionized the field of transcriptomics, offering unprecedented insights into gene expression patterns across entire genomes. This powerful technique allows researchers to quantify RNA levels, discover novel transcripts, and identify differentially expressed genes under various conditions. Whether you’re studying cancer progression, developmental biology, or environmental responses in organisms, RNAseq is an…
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Categories
- ATAC-seq (2)
- bulk RNA-seq (27)
- chromatin accessibility (14)
- Database (4)
- Epigenetics (14)
- Genomics (11)
- HPC (6)
- Metagenomics (1)
- Quick Tips (1)
- RNA-seq (20)
- Scientific Programming (6)
- Single Cell Sequencing (22)
- Transcriptomics (28)
Recent Posts
- How to Analyze Single-Cell ATAC-seq Data — A Complete Beginner’s Guide Part 2: Thorough Quality Control with Signac
- How to Analyze Single-Cell ATAC-seq Data — A Complete Beginner’s Guide Part 1: From FASTQ to Peaks
- How to Choose the Best Genome Aligner for a Specific NGS Dataset — A Beginner’s Guide to Read Mapping Tools
- How to Set Up a Bulk RNA-seq Pipeline on an HPC Cluster — A Complete Beginner’s Guide to Nextflow and nf-core/rnaseq
Tags
Alternative Splicing Analysis ATAC-seq BAM ChIP-seq chromatin accessibility CNV DESeq2 Differential Expression edgeR FASTQ GATK Mutect2 gene expression heatmap HOMER HPC Isoform limma MACS2 MAF miRNA miRNA-seq MSigDB Normalization peak calling RNA-seq SLURM somatic mutations Transcript VCF whole genome sequencing