About

Welcome to my NGS data analysis tutorial site. I’m Dr. Lei Guo, a Computational Biologist at UT Southwestern Medical Center with over a decade of experience in bioinformatics and molecular biology.

My Mission

In this information-driven era, my goal is to empower non-programmer biologists, medical doctors, and researchers to understand and perform NGS data analysis. Having collaborated with hundreds of wet lab researchers, I’ve gained unique insights into the challenges faced by bench scientists and clinicians when dealing with complex genomic data.

Why This Site Exists

  1. Bridge the Gap: I aim to translate complex computational concepts into language that resonates with biological and medical professionals.
  2. Empower Research: By understanding NGS analysis, you can better interpret your data, design experiments, and collaborate with bioinformaticians.
  3. Foster Independence: For those interested in hands-on analysis, I provide step-by-step tutorials to help you start your journey into bioinformatics.

What Sets My Tutorials Apart

  1. Extensive Collaboration Experience: Working with many wet lab researchers has given me a deep understanding of the questions and challenges non-programmers face when approaching NGS data.
  2. Broad NGS Expertise: My experience covers RNA-seq, scRNA-seq, spatial transcriptomics, ChIP-seq, ATAC-seq, methylation analysis, and more, allowing me to offer comprehensive guidance across various NGS technologies.
  3. Interdisciplinary Approach: With a background spanning computational biology, molecular biology, and cell biology, I provide insights that connect wet-lab experiments with computational analysis.
  4. Real-World Applications: My contributions to over 35 peer-reviewed publications in high-impact journals demonstrate the practical application of the techniques I teach.
  5. Focus on Accessibility: Drawing from my teaching experience, I break down complex concepts into digestible, learner-friendly content tailored for those without programming backgrounds.

Who Can Benefit

  • Biologists looking to understand their NGS data better
  • Medical doctors interested in genomic research
  • PhD students and postdocs venturing into bioinformatics
  • Researchers wanting to communicate more effectively with their computational collaborators
  • Anyone curious about the power of NGS data analysis in advancing biological and medical research

Whether you’re looking to understand the basics of NGS data analysis or aiming to perform your own analyses, my tutorials provide a clear pathway. From demystifying jargon to offering practical, step-by-step guides, I’m here to support your journey into the world of genomic data analysis.

Explore the tutorials, and don’t hesitate to reach out with questions or suggestions. Together, let’s unlock the potential of your NGS data and advance your research in this exciting informational era!

Connect with Me